High Diversity of Tick-associated Microbiota from Five Tick Species in Yunnan, China

Objective: Ticks are obligate blood-sucking vectors for multiple zoonotic diseases. In this study, tick samples were collected from Yunnan Province, China, which is well-known as the “Global Biodiversity Hotspot” in world. This study aimed to clarify microbial populations, including pathogens, associated with ticks and identify diversity of tick-borne microbiota region. Methods: The 16S rRNA full-length sequencing pooled DNA PCR amplification pathogenic genera individual performed understand tick-associated Results: A total 191 adult 5 species included revealed 11 phyla 126 bacteria, Anaplasma , Ehrlichia Candidatus Neoehrlichia, Rickettsia Borrelia Babesia . Further identification suggested that sp. YN01 was a variant strain spp. IG-1, but YN02 YN03, potentially two new SFGR species. Conclusions: complexity ecological interactions between host microbe provided insight biological control ticks. high identified, more investigation should be undertaken elucidate pathogenicity area.